Table 1. PCR Primers and conditions used in this study.

Primer name	Sequence (5’ to 3’)	Expected PCR product size (bp)	PCR cycle parameters
BBE02Nde-long	CGC ATA TGA AAA CTA ATG ATA TCG TAA		95°C for 1 min
	AAA CAA ATA AT		50°C for 1 min
BBE02Xho-long	CGC TCG AGT TAT TTA TGA TAA AAA ATT	3834	72°C for 4 min
	TTA TTA TTT AGT AAA TAA TTA TC
flaBpro5XbaI	TCT AGA CCG ATC GCC CTT CCC AAC AGT		95°C for 1 min
	TGC GCA GTG GAA GG		50°C for 1 min
BblucPacI	CGG TTA ATT AAT TAT TAT ACA GCA ATT TTA CCA CCT	1910	72°C for 2 min
5FlgBpro	GCT TAA TTA ACT TTT TTT TGA AGT GCC TGG		95°C for 45 sec
	CAG TAA GTT G		55°C for 45 sec
3aadA	GCT TAA TTA ATT ATT TGC CGA CTA CCT TGG	939	72°C for 1 min
	TGA TC		95°C for 30 sec
5BBE02mut	AAG ATA TCT AAA GAC AAG TAA TGT AAT	2225 or 3831	66°C for 30 sec
	AGA G		72°C for 4 min
3BBE02mut	CAT CGT CTT TAC TAT CTT TCA AAT CC		95°C for 30 sec
5BBQ67	AAT ATG TTT CAT TGT TTT ATA TCT TGG CTC	1407	60°C for 30 sec
3BBQ67	CCC TAT TGT TAG TTT TAT TGT TAG TAG TTT		72°C for 2 min