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. 2015 Jun 12;10(6):e0129532. doi: 10.1371/journal.pone.0129532

Table 1. PCR Primers and conditions used in this study.

Primer name Sequence (5’ to 3’) Expected PCR product size (bp) PCR cycle parameters
BBE02Nde-long CGC ATA TGA AAA CTA ATG ATA TCG TAA 95°C for 1 min
AAA CAA ATA AT 50°C for 1 min
BBE02Xho-long CGC TCG AGT TAT TTA TGA TAA AAA ATT 3834 72°C for 4 min
TTA TTA TTT AGT AAA TAA TTA TC
flaBpro5XbaI TCT AGA CCG ATC GCC CTT CCC AAC AGT 95°C for 1 min
TGC GCA GTG GAA GG 50°C for 1 min
BblucPacI CGG TTA ATT AAT TAT TAT ACA GCA ATT TTA CCA CCT 1910 72°C for 2 min
5FlgBpro GCT TAA TTA ACT TTT TTT TGA AGT GCC TGG 95°C for 45 sec
CAG TAA GTT G 55°C for 45 sec
3aadA GCT TAA TTA ATT ATT TGC CGA CTA CCT TGG 939 72°C for 1 min
TGA TC 95°C for 30 sec
5BBE02mut AAG ATA TCT AAA GAC AAG TAA TGT AAT 2225 or 3831 66°C for 30 sec
AGA G 72°C for 4 min
3BBE02mut CAT CGT CTT TAC TAT CTT TCA AAT CC 95°C for 30 sec
5BBQ67 AAT ATG TTT CAT TGT TTT ATA TCT TGG CTC 1407 60°C for 30 sec
3BBQ67 CCC TAT TGT TAG TTT TAT TGT TAG TAG TTT 72°C for 2 min