Table 3. Real-time PCR quantitation of B. burgdorferi genomic copy number at four weeks of infection in the tissues of mice inoculated with N40 bbe02::Bbluc-aadA.
| Inoculum | Mouse | Copies of B. burgdorferi genomic DNA per 200 ng of mouse DNA | |
|---|---|---|---|
| Ear | Joint | ||
| 10 | I | ND | 139.74 |
| 10 | II | ND | ND |
| 10 | III | ND | ND |
| 102 | I | ND | 149.24 |
| 102 | II | ND | ND |
| 102 | III | 137.55 | ND |
| 103 | I | ND | 94.39 |
| 103 | II | 110.45 | 60.06 |
| 103 | III | 47.55 | 52.72 |
| 103 | IV | 98.63 | 26.81 |
Data normalized to obtain B. burgdorferi copy number in 200 ng of DNA (105 copies of mouse nidogen gene) in respective tissues. ‘ND’ DNA not detected, i.e., below the detection limit.