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. 2015 Jun 12;10(6):e0128807. doi: 10.1371/journal.pone.0128807

Fig 5. Direct comparison of the activity of SAR405838 in SJSA-1 parental and representative sublines obtained from either in vitro or in vivo treatment with SAR405838.

Fig 5

A, Cell growth inhibition of SAR405838 in SJSA-1 parental and representative in vitro and in vivo sublines. Cells were treated with SAR405838 for 4 days and cell viability was evaluated by a WST assay. B, Analysis of apoptosis and cell cycle in SJSA-1 parental and representative in vitro and in vivo sublines. Cells were treated with SAR405838 for 48 h for apoptosis analysis by flow cytometry with Annexin V/P.I. double staining. The test was used to compare sublines (G2M1, G2M2, G2M4-G2M8) to 7.6 subline. *, P < 0.05. Cells were treated with SAR405838 for 24 h for cell cycle analysis by flow cytometry with P.I. staining. C, Western blot analysis of p53, MDM2, p21 and GAPDH protein level in SJSA-1 parental, and representative in vitro and in vivo sublines. Cells were treated for 24 hr with SAR405838 at indicated concentrations.