Skip to main content
. 2015 May 23;6(7):623–631. doi: 10.7150/jca.11291

Figure 4.

Figure 4

Effect of PD on MAPK signaling pathways in NCI-H460 and A549 cells. (A and B) NCI-H460 and A549 cells treated with 0, 5, 10, 20 and 30 µmol/L of PD for 24 h or 30 µmol/L of PD for 0, 3, 6, 12 and 24 h were analyzed by western blot with antibodies against p-Erk1/2, p-JNK and p-p38 MAPK. (C and D) NCI-H460 and A549 cells treated with 20 µmol/L of SB203580, 20 µmol/L of U0126 or 20 µmol/L of SP600125 for 4 h followed by treatment with or without 30 µmol/L of PD for 24 h were analyzed by western blot with antibodies against p-Erk 1/2 , p-JNK, p-p38 MAPK and LC3-І /II. (E and F) Densitometry analysis of LC3-II levels relative to LC3-І in NCI-H460 and A549 cells was performed. Representative results of three independent experiments are shown. β-actin was used as a loading control. Error bars, SD; □□: P<0.01 and □□□: P<0.001: versus PD + SB203580 values; ○○: P<0.01 and ○○○: P<0.001 versus PD + U0126 values; **: P<0.01 and ***: P<0.001 versus PD + SP600125 values.