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Amino acid Alignment of the MmedIR25a.1/25a.2 with IR25a sequences in other insects. Black shadings indicate amino acids which show at least 75% identity between sequences. Positions of the amino-terminal domain (ATD), the ligand-binding domain lobes (S1 and S2), the pore loop (P) and the transmembrane segments (M1, M2, M3) are marked with different colors bars according to their position in DmelIR25a. Amel, A. melifera; Dmel, D. melanogaster; Mmed, M. mediator; Nvit, N. vitripennis.
