Fig 4.

JNK mediated bFGF-induced dermal fibroblast migration under diabetic conditions. (A) JNK inhibitor SP600125 (10 µM) suppressed bFGF-stimulated dermal fibroblast migration at 12 or 24 h after wounding, as detected through a wound-healing assay. (B, C) The migration rate of dermal fibroblast in the presence of SP600125 is expressed as the migrating distance per hour and analyzed. The data are represented as the means ± S.E.M. from six independent experiments. (D, E) bFGF increased the activity of JNK but was markedly inhibited by SP600125, as detected by western blot. *P < 0.05 and **P < 0.01 compared with the indicated control group.