Fig 6. Sensitivity analysis of the model parameters.

Percentage changes in Ca²⁺ wave (A) peak amplitude, (B) time to peak, (C) Ca²⁺ wave width at half maximum, and (D) end Ca²⁺ concentration at 90 seconds’ time point due to changes in model parameters $k_{I{P}_3{R}_3}$, k_RyR, V_Pump, K_Pump, J_Leak, θ, L, α₄, $I{n}_{I{P}_3}$, $I{n}_{C{a}^{2+}}$, $D_{I{P}_3}$, and $D_{C{a}^{2+}}$as marked in the x-axis. The analysis was carried out in NB layers NB1, NB5 and NB10 as denoted in (A) for parameter $k_{I{P}_3{R}_3}$. The amount of modification (-25%, -10%, +10% or +25%) is shown in the grayscale of the histogram. The histogram bar is marked with asterisk (*) if the change was greater than 50%, and with double asterisk (**) if parameter modification did not result typical Ca²⁺ waveform. Regarding each Ca²⁺ wave feature, the parameter is illustrated only if the change was more than 5%.