Fig 1.

Pim-2 is highly expressed in colorectal tumor cells. (A) Images showing the Pim-2 expression pattern in cultured HCT116 human colorectal tumor cells. Green fluorescence indicates Pim-2, and blue indicates DAPI. Bar 25 μm. (B) Real-time PCR results showing that Pim-2 mRNA levels were significantly high in colorectal tumor cells. Results are the average of four independent experiments. Data represent mean ± SEM. ***p<0.001. (C-D) Western blots and histograms showing that the Pim-2 protein level was reduced by starvation and restored by re-feeding in HCT116 cells. Results are the average of four independent experiments. Data represent mean ± SEM **p<0.01.