Fig 5.

Inhibition of mTORC1 signaling by rapamycin reduces Pim-2 mediated glycolysis. (A) Western blots showing that rapamycin treatment inhibited mTORC1 activity under both basal and HA-Pim-2 overexpression conditions in NCM460 cells. (B-C) Biochemical results showing that rapamycin treatment restored Pim-2 induced ATP (B), glucose consumption, pyruvate and lactate production (C) in NCM460 cells. Results are the average of four independent experiments. Data represent mean ± SEM *p<0.05 and **p<0.01. N.S., not statistically significant.