Figure 4. Increased cell proliferation in cells transfected with wild-type or mutant EPHB4.

H661 cells were transfected with either wild-type or mutant EPHB4 constructs, and their effect on cell proliferation was measured over the time points shown using resazurin fluorescence. Untransfected and mock-transfected (transfection reagent only) cells served as controls, and data points represent the percent versus matched mock-transfected cell values at each time point. Each condition was repeated in eight replicates. Error bars indicate SEM. Overall p values shown were calculated by two-way ANOVA for time and construct.