Skip to main content
. 2015 Mar 2;6(9):6597–6610. doi: 10.18632/oncotarget.3260

Figure 6. Combined Akt inhibition increases apoptosis.

Figure 6

A Western blot analysis documenting the increase of PARP or of caspase-3 cleavage in MOLT-4, JURKAT and CEM-S cell lines, after 24 h of multiple drugs administration when compared with single exposure. Antibody to β-actin served as a loading control. B Flow cytometric analysis of MOLT-4, JURKAT and CEM-S cell lines treated with Perifosine, GSK690693 and MK-2206 administered alone and in combination for 24 h is reported. Triple drug exposure was also performed in the presence of 50 μM Z-VAD-fmk, a pan-caspase inhibitor. In the table are reported the percentages of annexin V positive cells after each treatment. Samples were incubated with Annexin V-fluorescein isothiocyanate. Results are the mean of three different experiments ± SD. Asterisks indicate statistically significant differences (*p < 0.05). One representative experiment of three is shown as well as cell lines are representative also of the others not shown.