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. 2015 Feb 3;6(9):6656–6669. doi: 10.18632/oncotarget.3169

Figure 4. S100A6 was co-expression and co-localization with CXCL14 in cells and the subcutaneous xenograft tumor tissues.

Figure 4

(A) The protein expression of CXCL14 in cell in two normal kidney cell lines: HKC and HK-2, as well as four ccRCC cell lines: 769-P, 786-O, Caki-1 and SN12-PM6 were detected by Western blot analysis. (B) The protein expression of CXCL14 of different transfected S100A6: the overexpression and knockdown of S100A6, together with each empty vector of 786-O were also detected by Western blot analysis. (C) The expression and location of S100A6 and CXCL14 were analyzed by immunofluorescence staining in 786-O cell line. (D) The expression and location of S100A6 and CXCL14 were analyzed by immunohistochemistry in the subcutaneous xenograft tumor tissues of shS100A6- and shControl-injection groups. CXCL14 was strongly stained in the tissues which were developed from the shS100A6 cells.