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. 2015 Feb 18;6(9):6776–6793. doi: 10.18632/oncotarget.2882

Figure 4. CAXII increases during the acquisition of chemoresistance.

Figure 4

HT29 cells were cultured in medium containing increasing concentrations of doxorubicin, as detailed under Methods. (A–C) At passage (P) 1, 5, 10, 15, 20 the mRNA was extracted and the expression of HIF-1α (panel A), CAXII (panel B) and Pgp (panel C) was detected by qRT-PCR. Data are presented as means ± SD (n = 4). Versus P1: *p < 0.001. (D) An aliquot of cells was incubated 24 h with 5 μmol/L doxorubicin, then lysed and analyzed for the intracellular doxorubicin content. Data are presented as means ± SD (n = 4). Versus P1: *p < 0.001.