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. 2015 Feb 4;6(9):7011–7022. doi: 10.18632/oncotarget.3137

Figure 1. Upregulation of TNKS1BP1 by ionizing radiation (IR) and its effect on the radiosensitivity of cells.

Figure 1

(A) Immunoblotting hybridization showing the increased expression of TNKS1BP1 protein in HeLa cells at given times after exposed to 4 Gy of γ-rays. (B) Immunoblotting hybridization showing the increased expression of TNKS1BP1 protein in MCF7, HepG2 and L02 cells at 4 h after different doses of γ-rays. (C) Increased TNKS1BP1 protein levels in both cytoplasm and nuclei in HeLa cells at 4 h after 4 Gy of γ-rays. (D) Quantitative real-time RT-PCR analysis showing the increased mRNA expression of TNKS1BP1 in HeLa cells at 2 h after 4 Gy of γ-rays. (E) Depletion of TNKS1BP1 expression in HeLa cells mediated by specific shRNA. (F) Depletion of TNKS1BP1 expression in HepG2 cells mediated by specific shRNA. (G) TNKS1BP1 depleted HeLa-shTNKS1BP1 cells became much more sensitive to IR as compared to the control HeLa-NC cells. (H) TNKS1BP1 depleted HepG2-shTNKS1BP1 cells became much more sensitive to IR as compared to the control HepG2-NC cells.