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. 2015 Jan 23;6(9):7136–7150. doi: 10.18632/oncotarget.3044

Figure 1. Rapamycin inhibits the basal or IGF-1-stimulated cell adhesion.

Figure 1

Rh30, Rh1, HT29 and HeLa cells were treated with or without rapamycin (Rapa, 100 ng/ml) in the presence or absence of IGF-1 (10 ng/ml) for 1.5 h following pre-incubation with rapamycin for 2 h, respectively. (A) Adherent cells were determined using CN IV-, fibronectin- or laminin-coated cell adhesion assay, and (B) cell viability was evaluated by MTS assay, as described in Materials and Methods. Results are means ± SE (n = 4–12). *P < 0.05, **P < 0.01, difference versus control group. ##P < 0.01, difference versus IGF-1 group.