Figure 4. Nexrutine^R modulates melanoma cell growth homeostasis.

(A) Effect of Nexrutine^R (10 μg/ml; 72 h) on melanoma cell viability (% of control) with and without NAC pre-treatment (5 mM; 1 h) determined by trypan blue exclusion assay. (B) Effect of Nexrutine^R on HEMn cell viability (live cells, % of control), determined by trypan blue exclusion assay. (C) Effect of increasing concentrations of Nexrutine^R on cell proliferation, percentage calculated based on vehicle treated controls. (D) Nexrutine^R inhibits soft-agar colony formation in melanoma cells. Percent colonies based on fluorescence values, normalized to vehicle controls. (E) Effect of Nexrutine^R (0, 20 μg/ml; 24 h) on melanoma cell apoptosis, determined using Annexin-APC and FACS. Significance values; *indicates p ≤ 0.05; **indicates p ≤ 0.01 and ***indicates p ≤ 0.001.