Figure 5. Nexrutine^R inhibits PI3K/AKT/mTOR signaling through oxidative stress modulation.

(A) Effect of increasing doses of Nexrutine^R treatment for 18 h on total and phospho-protein levels of the PI3K/AKT/mTOR pathway in WM793B, 1205Lu, and MeWo cells, determined by western blotting. β-actin was used as a loading control for each blot. The image is a representative of a minimum of 3 independent experiments. (B) Abrogation of inhibitory effect of Nexrutine^R on proteins downstream of mTORC1 with NAC pre-treatment (5 mM; 1 h). β-actin was used as a loading control for each blot.