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. 2015 Jan 31;6(9):7221–7231. doi: 10.18632/oncotarget.3372

Figure 2. ISG15 inhibits MDA-MB-231 breast tumor growth in nude mice and induces NK cell filtration into xenograft tumors.

Figure 2

A, ISG15 expression in MDA/LV-control and ISG15 shRNA was analyzed using anti-ISG15 antibodies in Western analysis as described in Methods. B, MDA/LV-control (Group I) and ISG15 (Group II) shRNA xenografts were established in female nude mice. On the same day, recombinant free ISG15 (188 ug) (Boston Biochemical) was injected near the site of xenograft implantation in Group III. Tumor volume was measured from 0-3 weeks after implantation as indicated in Methods. Each point represents average volume (+/− SEM) calculated from eight different tumors for Group I and II, and four different tumors for Group III. Representative photographs of tumor-bearing mice after three weeks of all test groups are shown in the right panel. C, Frozen tumor sections (from Group I, II, and III shown in A) were immunostained using anti-CD49b-HHMα2 antibodies as described in Methods.