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. 2015 Jan 23;6(9):7280–7292. doi: 10.18632/oncotarget.3110

Figure 2. Expression of apoptosis regulatory proteins and DRs, and NF-κB activity in human cervical tissues.

Figure 2

The expression of apoptotic proteins was detected by Western blotting using specific antibodies; DR3, DR6, FAS, p53, Bcl-2, cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, Bax and β-actin in the cervical cancer tissues and cervical normal tissues (A). β-actin protein was used an internal control. Immunohistochemistry analysis of FAS, DR3 and DR6 confirmed that the intensities of nuclear staining for DR3, DR6 and FAS were decreased in the cervical tissues (containing tumor tissues) of human (B). Activation of NF-κB in the cervical cancer tissues and cervical normal tissues (C). Nuclear extract from cervical cancer tissues was incubated in binding interaction of 32P-end-labeled oligonucleotide containing the κB sequence. The cervical tissues was incubated and were lysed, cytosolic proteins were used to determine the expression of IκB, p-IκB (internal control) and nuclear proteins were used to determine the expression of p50, p65 and Histone-H1(internal control) in cervical tumor tissues.