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. 2015 Jan 23;6(9):7280–7292. doi: 10.18632/oncotarget.3110

Figure 5. Effect of BV on apoptotic cell death.

Figure 5

The cervical cancer cells were treated with BV for 24 hr, and then labeled with DAPI and TUNEL solution. Total number of cells in a given area was determined by using DAPI nuclear staining (fluorescent microscope). The green color in the fixed cells marks TUNEL-labeled cells. The apoptotic index was determined as the DAPI-stained TUNEL-positive cell number/total DAPI stained cell number (magnification, 200 ×). Values were means ± S.D. of three experiments. *(P < 0.05) indicates statistically significant differences from the control cells.