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. 2015 May 26;112(23):7237–7242. doi: 10.1073/pnas.1505924112

Fig. 4.

Fig. 4.

The pH-dependent recognition of apoptotic and necrotic cells by DEC205. (A) DEC205-GFP binds to the ActD-treated Jurkat cells at acidic pH. (B) DEC205-GFP binds to the Apopida-treated HEK293 cells at acidic pH. (C) DEC205-GFP binds to the frozen-thawed HEK293 cells at acidic pH. (D) Triple staining of the viable Jurkat cells (Left) and the ActD-treated Jurkat cells (Middle) by Annexin V-APC, PI, and DEC205-GFP at acidic pH. The binding of DEC205 to the gated subsets of the viable and the ActD-treated Jurkat cells are shown on the right. (E) CysR∼CTLD3-GFP (the small ring) binds to the ActD-treated Jurkat cells at acidic conditions. (F) FNII∼CTLD6-GFP (the large ring) does not bind to the ActD-treated Jurkat cells at either acidic or basic conditions. (G) DEC205 (H129E)-GFP does not bind to the ActD-treated Jurkat cells at either acidic or basic conditions. (H) The fixed and permeabilized HEK293 cells stained by DEC205-GFP and DAPI at pH 6.0 by confocal microscopy. (I) The fixed and permeabilized HEK293 cells stained by DEC205-GFP and DAPI at pH 7.4 by confocal microscopy. (Scale bars, 25 μm.) (J) A cartoon representation of the pH-dependent recognition of apoptotic cells by DEC205.