Phenotype of etiolated A. thaliana wild-type and Atwscp knockout seedlings. (A) Schematic presentation of the AtWSCP gene including the 5′ and 3′ untranslated regions, position of the T-DNA insertion in the single exon (triangle), and primers used to prove the presence of the T-DNA (R, F, and GT2). (B) Genotyping of the Atwscp mutant with the indicated gene-specific primers. (C) Western blot with the polyclonal WSCP antibody. (D) Greening of 3-d-old etiolated wild-type and Atwscp mutant seedlings after 3, 6, or 24 h of white light exposure.