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LAF-1 depletion disrupts P granule organization. (A) The percentage of hatched embryos laid by singled hermaphrodites (n = 42) is strongly decreased in laf-1(RNAi) mothers. (B) Estimation of LAF-1 concentration in the germplasm as a function of laf-1(RNAi) exposure. (C–E) Epifluorescent images of four-cell embryos immunostained for LAF-1 (C), PGL-1 (D), and PIE-1 (E) at 30 h of RNAi feeding. (Scale bars, 10 μm.)
