Table S3.
Average Kd values of RNAP binding to Cy3-labeled ssDNA (−12 to +1) with and without RbpA
| Promoter | Sequence | No RbpA Kd (μM) | RbpA Kd (μM) |
| Consensus | *TATAATGGGAAGG | 0.38 ± 0.07 | 0.33 ± 0.04 |
| AP3 | *TAGACTGGCAGGG | 2.24 ± 0.41 | 1.88 ± 0.31 |
| vapB | *TATGATATGGTGT | 1.62 ± 0.26 | 1.77 ± 0.15 |
Average Kd values of RNAP binding to Cy3-labeled ssDNA (−12 to +1) were determined using fluorescence anisotropy. The addition of RbpA to the assay has no significant effect on RNAP binding to ssDNA composed of the −10 and discriminator elements; −10 element is bold. Error is based on triplicate trials.