Fig. 1.
Schematics of the lysogenizing phages. The CRISPR-associated genes cas3, cse1, cse2, cas7, cas5, and cas6e (blue) were inserted in place of nucleotides at position 19,014–27,480 of the λ chromosome (National Center for Biotechnology Information Reference Sequence: NC_001416.1) yielding the control lysogenizing phage λcas (Lower). The λcas-CRISPR phage (Upper) encodes, in addition to the cas genes, a CRISPR array with spacers targeting the genes ndm-1 (N1, N2, N3) and ctx-M-15 (C1, C2, C3). PT7, T7 promoter.