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. 2015 May 20;112(23):7291–7296. doi: 10.1073/pnas.1415845112

Fig. 4.

Fig. 4.

Activation of direct projections from the BLA to the dorsal hippocampus is sufficient to reproduce repeated stress-induced hippocampal molecular changes and learning and memory deficits. (A) Effect of BLA terminal photostimulation on p25 expression level, measured by Western blot analysis from hippocampal lysates (unpaired t test). (B and C) Representative immunohistochemical images and quantitative analysis of HDAC2 and Synaptophysin (B) and GR and pGR (C) expression levels in dorsal CA1 after BLA terminal photostimulation in the dorsal hippocampus (n = 5 per group; unpaired t test for HDAC2 and Synaptophysin, one-way ANOVA with Tukey’s post hoc analysis for GR/pGR) (pGR and HDAC2 in red; GR and Synaptophysin in green; DAPI in blue). (D) Effect of dorsal BLA terminal photostimulation on novel object (NO) and novel location (NL) recognition behavioral tasks (one-tailed t test). n = 16 for eYFP and 10 for ChR2. Values are mean ± SEM. n.s., nonsignificant; P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. (Scale bars: 20 µm.)