Fig. 5.

p25 generation is necessary for repeated stress-induced hippocampal molecular changes and learning and memory deficits. (A and B) Absence of RFS-induced changes in HDAC2 and Synaptophysin (A) and GR and pGR (B) expression levels in the hippocampal CA1 subregion in the Δp35KI hippocampus (n = 4 per group; one-way ANOVA Tukey’s post hoc analysis) (HDAC2 and pGR in red; Synaptophysin and GR in green; DAPI in blue). (C) Absence of repeated stress-induced learning and memory deficits in the Δp35KI mouse. (n = 8 for WT CON and RFS; n = 10 and 11 for ∆p35 CON and RFS, respectively; one-sample one-tailed t test). Values are mean ± SEM. n.s., nonsignificant; P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. (Scale bars: 20 µm.)