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. 2015 May 26;112(23):7327–7332. doi: 10.1073/pnas.1502076112

Fig. S2.

Fig. S2.

Effects of miR-200f and glycogene silencing on ZEB1. (A) miR-200b has a more pronounced effect than mir-200a on EMT. MDA-MB-231 cells were treated with scramble, miR-200a, or miR-200b mimics (50 nM, 7 d) and analyzed by qRT-PCR. qRT-PCR data for ZEB1 are reported as the average of relative expression of four biological replicates normalized to GAPDH. Error bars denote SD; *P < 0.005, t test. (B) Effect of glycogene silencing on ZEB1 Levels. Data are from MDA-MB-231 cells treated with control shRNA or shRNAs silencing (KO) B3GLCT, ST3GAL5, or ST6GALNAC5 on day 5 (control, B3GLCT, and ST3GAL5), or day 10 (ST6GALNAC5) after treatment. qRT-PCR data are reported as the average of relative expression of three biological replicates normalized to GAPDH. Error bars denote SD; *P < 0.05.