Table S4.
Expression profile of glycogenes not predicted to be targets of the miR-200 family in MDA-MB-231 cells treated with miR-200a, miR-200b, or scramble mimics
| miR-200a treatment | miR-200b treatment | ||||
| Glycogene | Fold change | P value | Glycogene | Fold change | P value |
| A4GNT | −1.41 | 7.2E-03 | A4GNT | −1.81 | 1.1E-03 |
| AGA | −1.37 | 2.5E-03 | AGA | −1.17 | 0.24 |
| B3GNT3 | 13.51 | 5.8E-06 | B3GNT3 | 67.80 | 1.4E-05 |
| B3GNT8 | −1.14 | 0.42 | B3GNT8 | 1.01 | 0.95 |
| B4GALT1 | −1.17 | 0.20 | B4GALT1 | −1.15 | 0.16 |
| B4GALT2 | 1.01 | 0.97 | B4GALT2 | −1.02 | 0.92 |
| B4GALT3 | 1.21 | 0.43 | B4GALT3 | 1.32 | 0.19 |
| B4GALT5 | 1.11 | 0.62 | B4GALT5 | 1.61 | 2.1E-02 |
| C1GALT1C1 | −1.01 | 0.94 | C1GALT1C1 | 1.02 | 0.80 |
| EDEM1 | −1.07 | 0.59 | EDEM1 | −1.05 | 0.65 |
| EDEM2 | −1.06 | 0.60 | EDEM2 | 1.04 | 0.66 |
| EDEM3 | 1.02 | 0.79 | EDEM3 | −1.10 | 0.23 |
| FUCA1 | −1.09 | 0.31 | FUCA1 | −1.12 | 0.19 |
| FUCA2 | −1.09 | 0.24 | FUCA2 | −1.03 | 0.61 |
| FUT8 | 1.20 | 0.19 | FUT8 | −1.07 | 0.45 |
| FUT11 | 1.03 | 0.79 | FUT11 | 1.28 | 0.12 |
| GALNT1 | 1.15 | 0.29 | GALNT1 | −1.12 | 0.30 |
| GALNT6 | 2.14 | 3.7E-04 | GALNT6 | 1.79 | 9.9E-05 |
| GALNT7 | −1.07 | 0.60 | GALNT7 | −1.09 | 0.39 |
| GALNT8 | −1.69 | 0.21 | GALNT8 | −2.07 | 0.18 |
| GALNT9 | −2.19 | 0.12 | GALNT9 | −1.90 | 0.17 |
| GALNT13 | −1.16 | 2.4E-02 | GALNT13 | 1.03 | 0.63 |
| GALNTL1 | −1.61 | 1.3E-02 | GALNTL1 | −2.02 | 3.3E-03 |
| GALNTL5 | 1.41 | 0.69 | GALNTL5 | 1.08 | 0.94 |
| GALNTL6 | −2.06 | 0.12 | GALNTL6 | −3.08 | 4.8E-02 |
| GANAB | −1.01 | 0.92 | GANAB | −1.02 | 0.80 |
| GCNT1 | −1.61 | 8.1E-05 | GCNT1 | −1.63 | 4.0E-03 |
| GCNT3 | −1.18 | 0.35 | GCNT3 | 1.49 | 0.23 |
| GLB1 | −1.06 | 0.73 | GLB1 | −1.17 | 0.26 |
| GNPTAB | −1.15 | 0.19 | GNPTAB | −1.03 | 0.84 |
| GNPTG | −1.15 | 0.26 | GNPTG | −1.13 | 0.22 |
| HEXA | −1.11 | 0.36 | HEXA | −1.14 | 9.1E-02 |
| HEXB | −1.13 | 0.24 | HEXB | −1.17 | 0.11 |
| MAN1A1 | 1.09 | 0.54 | MAN1A1 | −1.14 | 0.45 |
| MAN1A2 | 1.02 | 0.88 | MAN1A2 | −1.15 | 0.25 |
| MAN1B1 | −1.06 | 0.56 | MAN1B1 | −1.08 | 0.41 |
| MAN1C1 | −1.07 | 0.76 | MAN1C1 | −1.39 | 3.9E-02 |
| MAN2A1 | −1.06 | 0.23 | MAN2A1 | 1.13 | 0.23 |
| MAN2A2 | 1.24 | 0.41 | MAN2A2 | 1.43 | 0.12 |
| MAN2B1 | 1.04 | 0.89 | MAN2B1 | 1.01 | 0.97 |
| MANBA | −1.21 | 5.5E-04 | MANBA | −1.22 | 1.5E-02 |
| MGAT1 | 1.18 | 0.36 | MGAT1 | 1.37 | 1.9E-02 |
| MGAT4B | 1.13 | 0.39 | MGAT4B | 1.13 | 0.17 |
| MGAT5 | 1.27 | 0.32 | MGAT5 | 1.31 | 0.25 |
| MGAT5B | −1.29 | 0.15 | MGAT5B | −2.24 | 3.7E-03 |
| MOGS | 1.10 | 0.63 | MOGS | 1.32 | 9.2E-02 |
| NAGPA | −1.33 | 0.32 | NAGPA | −1.40 | 0.20 |
| NEU1 | −1.08 | 0.63 | NEU1 | 1.25 | 0.10 |
| NEU2 | 1.43 | 0.24 | NEU2 | 1.62 | 3.2E-02 |
| NEU3 | −1.05 | 0.63 | NEU3 | 1.24 | 7.4E-02 |
| NEU4 | −1.35 | 0.51 | NEU4 | −1.63 | 0.36 |
| OGT | −1.09 | 0.50 | OGT | −1.02 | 0.88 |
| POFUT2 | −1.14 | 0.18 | POFUT2 | −1.25 | 1.4E-02 |
| POMGNT1 | 1.06 | 0.71 | POMGNT1 | −1.05 | 0.59 |
| POMT1 | −1.18 | 0.33 | POMT1 | −1.07 | 0.61 |
| POMT2 | 1.07 | 0.66 | POMT2 | 1.02 | 0.85 |
| PRKCSH | 1.02 | 0.86 | PRKCSH | 1.07 | 0.39 |
| ST3GAL1 | 1.19 | 5.6E-02 | ST3GAL1 | 1.21 | 2.7E-02 |
| ST6GAL1 | −1.60 | 8.5E-03 | ST6GAL1 | −2.08 | 3.8E-04 |
| ST6GALNAC1 | 4.57 | 4.4E-02 | ST6GALNAC1 | 9.10 | 6.1E-03 |
| ST8SIA3 | −1.11 | 0.91 | ST8SIA3 | −1.06 | 0.95 |
| ST8SIA6 | −1.72 | 1.7E-02 | ST8SIA6 | −3.13 | 1.8E-03 |
| UGGT1 | −1.03 | 0.87 | UGGT1 | −1.15 | 0.30 |
| UGGT2 | −1.09 | 0.44 | UGGT2 | −1.18 | 9.3E-02 |
MDA-MB-231 cells were treated with miR-200a, miR-200b, or scramble mimics (50 nM) for 7 d. qRT-PCR was performed using RT2 Profiler PCR Array Human Glycosylation (Qiagen). Fold change (miR-treated/scramble-treated) was calculated by taking the average relative expression of miR-treated samples (n = 4 independent experiments, normalized to GAPDH) over the average relative expression of the scramble-treated cells (n = 4 independent experiments, normalized to GAPDH). Where the fold change was less than 1, the negative inverse is shown. P values for treated vs. scramble were calculated using Student's t-test. Statistically significant (P ≤ 0.05) down-regulation is in bold type, and statistically significant up-regulation is underlined.