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. 2015 Jun 12;10(6):e0129859. doi: 10.1371/journal.pone.0129859

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© 2015 Milewska et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A. Cos-1 total cell extracts were immunoprecipitated using an anti-BRAF antibody and immunoblotted with the indicated antibodies. B. Cellular extracts from Cos-1 were immunoprecipitated with anti-MIG-6 antibody followed by Western blot analysis detecting BRAF and MIG-6, respectively. C. Cos-1 cells were transiently transfected with plasmid DNA encoding FLAG fused to WT or mutant BRAF and co-transfected with HA-MIG-6. Whole cell extracts were subjected to anti-FLAG specific IP and blotted with the indicated antibodies. D. Densitometrical analysis of MIG-6 band intensity in anti-FLAG specific IPs. MIG-6 co-immunoprecipitation is reported as changes normalized to MIG-6 co-immunoprecipitating with FLAG-BRAF WT. Data represent the mean of three independent experiments ± SD. P values were calculated using paired Student`s t-test.