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. 2015 Jun 12;10(6):e0129859. doi: 10.1371/journal.pone.0129859

Fig 4. Oncogenic BRAF V600E Induces Expression of Endogenous MIG-6, which in turn Negatively Regulates the EGFR.

Fig 4

A. Cos-1, HEK293 and MCF-7 cell lines were transiently transfected with FLAG-BRAF WT or V600E expression plasmids. Total cell extracts were analyzed by Western blotting with the indicated antibodies. B. A431 cells were transiently transfected with expression vectors for BRAF WT, V600E or the impaired kinase activity mutant D594V. Total cell extracts were analyzed by immunoblotting with the depicted antibodies. C,D. The same experiment as in B except that treatment with 10μM U0126 MEK inhibitor for 30 minutes (panel C—lanes 2, 4, 6 and 8; panel D—lanes 2, 4, 6 and 8) and/or MIG-6 overexpression (panel D—lanes 5–8) was included. E. A431 cells were transiently transfected with FLAG-BRAF WT or V600E expression plasmids as indicated. Treatment with the MEK inhibitor U0126 (lanes 4 and 8) as well as concomitant transfection with either control (lanes 1–4) or MIG-6 siRNA (lanes 5–8) were included as additional conditions.