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. 2015 Jun 15;5:11282. doi: 10.1038/srep11282

Figure 5. The self-association competent and catalytically active Tgs1p is required to trimethylate snRNAs in the absence of Swm2p.

Figure 5

Total RNA from wild-type, or swm2Δ yeast strains transformed with either an empty PRS316-GFP vector, or a vector expressing yTgs1-FL or ytgs1Δaa1-10 was immunoprecipitated with anti-TMG antibodies and analysed by Northern blotting. Quantification of the immunoprecipitation efficiency as a fraction of input is presented in the table on the left. The total RNA input was set as 100%. Sup: supernatant.