| Plasmids |
|
|
| PRS316-GFP |
CEN, URA3, PPGK1-EGFP
|
12 |
| EGFP-yTGS1 |
CEN-URA3-PPGK1-EGFP-yTGS1
|
12 |
| EGFP-yTGS1-aa10-315 |
CEN, URA3, PPGK1-EGFP-yTgs1 (aa10-315)
|
This study, pMK452 |
| EGFP-yTgs1-aa40-315 |
CEN, URA3, PPGK1-EGFP-yTgs1 (aa40-315)
|
This study, pMK453 |
| EGFP-yTgs1-W178A |
CEN, URA3, PPGK1-EGFP-yTgs1 (W178A)
|
This study, pMK454 |
| NOP1-mRFP |
CEN-URA3-PNOP1-NOP1-mRFP
|
19 |
| pGADT7 |
2 μ, Leu2, G4AD-HA |
Clontech Laboratories, Inc. |
| pGBKT7 |
2 μ, TRP1, G4BD-c-myc |
Clontech Laboratories, Inc. |
| pVA3-1 |
aa 72-390 of murine p53 expressed from pAS1CYH2; 2 μ, TRP1, pADH1, N-terminal G4BD |
Clontech Laboratories, Inc. |
| pTD1-1 |
aa 87-708 of SV40 large T antigen expressed from pACT2; 2 μ, Leu2, pADH1, N-terminal G4AD |
Clontech Laboratories, Inc. |
| pGAD-yTgs1-FL |
pGADT7-based for production of yTgs1p (aa1-315) |
12 |
| pGAD-yTgs1-aa50-262 |
pGADT7-based for production of yTgs1p (aa50-262) |
12 |
| pGAD-yTgs1-aa263-315 |
pGADT7-based for production of yTgs1p (aa263-315) |
12 |
| pGAD-yTgs1-aa1-49 |
pGADT7-based for production of yTgs1p (aa1-49) |
12 |
| pGAD-yTgs1-aa200-315 |
pGADT7-based for production of yTgs1p (aa200-315) |
12 |
| pGAD-yTgs1-aa1-100 |
pGADT7-based for production of yTgs1p (aa1-100) |
12 |
| pGAD-yTgs1-aa10-315 |
pGADT7-based for production of yTgs1p (aa10-315) |
This study, pMK457 |
| pGAD-yTgs1-aa40-315 |
pGADT7-based for production of yTgs1p (aa40-315) |
This study, pMK458 |
| pGAD-hTgs1-FL |
pGADT7-based for production of Tgs1p (aa1-853) |
This study, pMK459 |
| pGAD-hTgs1-aa631-853 |
pGADT7-based for production of Tgs1p (aa631-853) |
This study, pMK460 |
| pGAD-SWM2 |
pGADT7-based for production of Swm2p |
This study, pMK463 |
| pGBK-yTgs1-FL |
pGBKT7-based for production of yTgs1p (aa1-315) |
This study, pMK464 |
| pGBK-yTgs1-aa10-315 |
pGBKT7-based for production of yTgs1p (aa10-315) |
This study, pMK465 |
| pGBK-yTgs1-aa40-315 |
pGBKT7-based for production of yTgs1p (aa40-315) |
This study, pMK466 |
| pGBK-hTgs1-FL |
pGBKT7-based for production of hTgs1p (aa1-853) |
This study, pMK467 |
| pGBK-hTgs1-aa631-853 |
pGBKT7-based for production of hTgs1p (aa631-853) |
This study, pMK468 |
| pGBK-SWM2 |
pGBKT7-based for production of Swm2p |
12 |
| pETDuet-1 |
Expression vector; ColE1 replicon, T7 promoters, Apr
|
Novagen |
| pETDuet-yTGS1 |
pETDuet-1-based for production of 6His-yTgs1p |
This study, pMK496 |
| pRSFDuet-1 |
Expression vector; RSF replicon, T7 promoters, Knr
|
Novagen |
| pRSFDuet-GST |
pRFSDuet-1-based for production of 6His-GST |
This study, pMK497 |
| pRSFDuet-GST-yTgs1 |
pRFSDuet-1-based for production of 6His-GST-yTgs1 |
This study, pMK498 |
| Yeast strains |
|
|
| BY4741 |
MATa, his∆1, leu2∆0, met15∆0, Ura3∆0
|
Euroscarf |
| tgs1Δ
|
tgs1::kanMX6, otherwise as BY4741 |
12 |
| swm2Δ
|
swm2::His3MX6, otherwise as BY4741 |
12 |
| Y2H strain PJ69-4A |
MATa, trp1-901, leu2-3,112, ura3-52, his3-200, gal4, gal80, GAL2-ADE2, LYS2::GAL1-HIS3, met2::GAL7-lacz |
14 |
