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. 2015 Jun 15;5:11231. doi: 10.1038/srep11231

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Copyright © 2015, Macmillan Publishers Limited

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(A) After 24 h reperfusion, liver necrosis and apoptosis were assessed by H&E and TUNEL staining methods, respectively. (B) The area of necrosis was analyzed. (C) Apoptotic cells were counted and expressed as a percentage of all hepatocytes. (D) After 6 h reperfusion, serum levels of AST and ALT were analyzed. (E) After 24 h reperfusion, prothrombin time (PT) was analyzed. Values are the mean ± SEM (n = 9 mice per group). ^*, p < 0.05 versus sham-operated mice; ^#, p < 0.05 and ^##, p < 0.01 versus AdLacZ-injected mice. AdBP-3, AdIGFBP-3; AdBP-3 ^GGG, AdIGFBP-3^GGG.