Figure 6.

Effects of dietary calcium on the expression of PLC-γ1, PIKE and EGFR in the oral epithelium. The tongue was removed from the mice described in Figure 1 and the tissue was fixed in formalin solution, embedded in paraffin blocks and sectioned for routine histological and immunohistochemical analysis using antibodies against PLC-γ1, PIKE and EGFR. Positive staining was shown in brown and counterstaining was shown in blue. The representative section shows the average expression levels of PLC-γ1, PIKE and EGFR in oral papilloma, SCC and normal epithelium. Quantitation of PLC-γ1, PIKE and EGFR in the cells is shown in the bar graph. The quantitation was obtained as described in Figure 3. The data are expressed as mean ± SD, *P < 0.05 (compared with the normal calcium diet group).