Selection transfectants and expression of MMP2, MMP9, MMP15, TGFβ, VEGF, P53 and PTTG in transiently transfected cells and controls. A. Transfection efficiency was detected with fluorescent microscopy (40 ×magnifications). B. Downregulated MMP14 mRNA efficiency was measured by real-time PCR in five interference plasmids. C. mRNA of relative factors was measured with real-time PCR. Results are presented as means ± SD from three independent data. *P < 0.05 was significant compared to control.