Figure 4.

Phenotypic alterations of carcinoma cells by KLFs. A: Quantitative analysis of KLF4 and KLF5 mRNA in oral carcinoma cell lines and normal keratinocytes (HaCaT). The relative fold of expression against HaCaT cells was depicted (n = 4). Bars = mean ± S.D. B: Expression of protein involved in the differentiation (E-cadherin, Involucrin), dedifferentiation (Fibronectin) and proliferation (Cyclin D₁, p21^Cip1/Waf1). KLF4 (*) and KLF5 (**) expression were probed by anti-HA antibody. C: Expression of E-cadherin, involucrin and fibronectin upon transfection of siRNA against KLF4 (siKLF4) and KLF5 (siKLF5). Endogenous KLF4 and KLF5 expression was probed with anti-KLF4 and -KLF5 antibodies. b-actin was used as an internal control.