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. 2015 Apr 1;8(4):3735–3741.

Figure 2.

Figure 2

Role of TMZ in H/R-treated H9C2 cells. The H9C2 cells were randomly assigned into three groups: Sham group, in which the cells were treated with 0μM TMZ for 48 h and then cultured under normal oxygenation conditions (5% CO2, and 95% air); H/R group, in which cells were treated with 0 μM TMZ for 48 h and then cultured under H/R conditions; and H/R+TMZ group, in which cells were treated with 10 μM TMZ for 48 h and then subjected to H/R treatment. (A) qRT-PCR assay determined the relative expression of miR-21. Protein expression of PTEN (B) and p-AKT (C) were examined by western blot analysis, respectively (aP<0.01 and bP<0.05 vs. sham group, cP<0.05 vs. H/R group).