Figure 3.

miR-21 involved the regulation of TMZ on PTEN and p-AKT expression in HR-treated H9C2 cells. The H9C2 cells were assigned into four groups: sham group, in which the cell were treated with 0 μM TMZ for 48 h and then cultured under normal oxygenation conditions (5% CO₂, and 95% air); H/R group, in which cells were treated with 0 μM TMZ for 48 h and then cultured under H/R conditions; and H/R+TMZ group, in which cells were treated with 10 μM TMZ for 48 h and then subjected to H/R treatment; H/R+TMZ+ inhibitors group, in which cells were transfected with 50 nM miR-21 inhibitors, next, treated with 10 μM TMZ for 48 h and finally subjected to H/R treatment. Protein expression of PTEN (A) and p-AKT (B) were examined by western blot analysis, respectively. ^aP<0.01 vs. sham group, ^bP<0.01 and ^cP<0.05 vs. H/R group, ^dP<0.01 vs. H/R+TMZ+NC group.