Table 2.
Designed PCR primers
| Name | Sequence | Product length |
|---|---|---|
| SOX9 | 5’-ACGGCTCCAGCAAGAACAAG-3’ | 269 bp |
| 5’-CCCGTTCTTCACCGACTTCC-3’ | ||
| MEK1 (MAP2K1) | 5’-AGCTCTGCGGAGACCAACTT-3’ | 233 bp |
| 5’-AGATGAATTAGCTTTCTGGCCA-3’ | ||
| MEK2 (MAP2K2) | 5’-TCAAGCTGTGTGACTTCGGG-3’ | 116 bp |
| 5’-CACCGAGTAATGTGTGCCCT-3’ | ||
| ERK1 (MAPK3) | 5’-GTCATCGGCATCCGAGACAT-3’ | 246 bp |
| 5’-CTTAAGGTCGCAGGTGGTGT-3’ | ||
| ERK2 (MAPK1) | 5’-GGCATGGTGTGCTCTGCTTAT-3’ | 399 bp |
| 5’-GCCAAAGTCACAGATCTTGAGATC-3’ | ||
| β-actin | 5’-CTGGCACCACACCTTCTACAATG-3’ | 248 bp |
| 5’-CCTCGTAGATGGGCACAGTGTG-3’ | ||
| PGL3-MEK1-wt | 5’-TGGGCAACTGAGGGAGACACCGT-3’ | 657 bp |
| 5’-TGGGAGGGACTGGAGGCCGG-3’ | ||
| PGL3-MEK1-del-1 | 5’-GTGACGTATTTCCGCGTCATCTGCCG-3’ | 160 bp |
| 5’-TGGGAGGGACTGGAGGCCGG-3’ | ||
| PGL3-MEK1-del-2 | 5’-AACTGTCGCCTCATCAGCACTG-3’ | 651 bp |
| 5’-CAGTGCTGATGAGGCGACAGTT-3’ | ||
| PGL3-MEK2-wt | 5’-AGGACGTGGCCCTCAGTGAAATATGCC-3’ | 877 bp |
| 5’-CCGAGGCCCGAAGAAGGCTGAC-3’ | ||
| PGL3-MEK2-del-1 | 5’-GAAAGGCGGCCTTGTGCTGCTG-3’ | 166 bp |
| 5’-CCGAGGCCCGAAGAAGGCTGAC -3’ | ||
| PGL3-MEK2-del-2 | 5’-AGGACGTGGCCCTCAGTGAAATATGCCAGCCGGACAAAAC-3’ | 871 bp |
| 5’-CCGAGGCCCGAAGAAGGCTGAC-3’ | ||
| PGL3-ERK2-wt | 5’-ACCTGATTGGCGTATCCTCTCAG-3’ | 588 bp |
| 5’-CCTGCCTGCCAGACTGAC-3’ | ||
| PGL3-ERK2-del-1 | 5’-CAGTCAACGCCGTCGCAGTG-3’ | 182 bp |
| 5’-CCTGCCTGCCAGACTGAC -3’ | ||
| PGL3-ERK2-del-2 | 5’-ACCTGATTGGCGTATCCTCTCAGTGTCTCCTTTTAGTCTCGTG-3’ | 583 bp |
| 5’-CCTGCCTGCCAGACTGAC-3’ |