Figure 1.

P120ctn protein expression paralleled with LPS-induced the decrease of blood-brain barrier (BBB) integrity and the expression of tight-junction proteins in HBMECs. Cells were stimulated with LPS (1 μg/mL) over a range of indicated time points. A. The expression of p120ctn was examined by western blotting using GAPDH as an internal control. B. The concentration of FITC-dextran in LPS was measured as described in methods section. C. Correlation between p120ctn expression and the FITC-dextran concentration. D. The expression of occludin and claudin-5 were analyzed by western blotting. E and F. P120ctn expression was positively correlated with the expression of occludin and claudin-5. Group (0 h) was considered as control group. All data were expressed as mean ± SEM. *P<0.05, **P<0.01 vs. control, n=6.