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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biotechniques. 2015 Jun 1;58(6):308–317. doi: 10.2144/000114299

Figure 2.

Figure 2

Recovery of RNA from RiboTag-expressing primary neuronal/glial co-cultures, and validation of housekeeping genes. qPCR analysis demonstrating average relative starting quantity of mRNA ± SEM. (a–b) Transgenic mRNA (mRuby2) (a) RiboTag-IP and (b) Input fraction. (c–d) Endogenous housekeeping gene mRNA (c) RiboTag-IP and (d) Input fraction. n=5–8. ANOVA, mRuby2: F(4, 30) (a) F=20.31 p<0.0001, (b) F=12.46 p=<0.0001. HPRT: F(4,31) (c) F=63.46 p<0.0001, (d) F=1.784 p=0.157. PPIA: F(4,31) (c) F=35.55 p<0.0001, (d) F=1.178 p=0.34. ß-actin: F(4,26) (c) F=14.1 p<0.0001, (d) F=0.9 p=0.48. GAPDH: F(4,26) (c) F=59.72 p<0.0001, (d) F=1.68 p=0.19. Bonferroni post-hoc, *p<0.05, **p<0.001 compared to CMV-RiboTag+CMV-mRuby2 and #p<0.05 compared to hSyn-mRuby2-2A-RiboTag.