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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Oct 18;32(12):3012–3023. doi: 10.1161/ATVBAHA.112.254219

Figure 4. Minimal macrophage recruitment and Mcp1 expression in the aorta of Notch1+/−;Apoe−/− mice after 7 and 28 days of AngII infusion.

Figure 4

Immunohistochemistry showing decreased expression of Moma2, a macrophage marker, in the aneurysmal tissue at day 7 and day 28 of AngII-treated Notch1+/−ApoE−/− mice (B, F, and D, H) as compared to ApoE−/− mice (A, E and C, G). Decreased Mcp1 expression in the abdominal aorta of Notch1+/−;ApoE−/− mice (J, N and L, P) in response to AngII as compared to ApoE−/− mice (I, M and K, O). Moma2 and Mcp1 expression was specifically decreased in adventitial layer (F, H, N, P; arrowheads). (Q) qRT-PCR demonstrates reduced mRNA levels of Mcp1, Il6, Cxcl10, Vegf, iNOS, Icam1 and Vcam1 in the abdominal aorta of Notch1+/−;Apoe−/− mice as compared to Apoe−/− mice treated with AngII for 28 days (n=3, in triplicate). (R) qRT-PCR demonstrates reduced expression of a similar panel of inflammatory mediators in LPS-stimulated primary macrophages isolated from the peritoneal cavity of Notch1+/−;Apoe−/− mice as compared to Apoe−/− mice. Macrophages from 5 mice were pooled for each experiment; data shown represents 3 experiments performed in triplicate. ***P<0.001; **P<0.01; *P<0.05. Means and standard deviations are shown. Scale bar, 50 μm.