Fig 3. Detection of Gag and Pol proteins in S2 cells and larval brain extracts.
(A) Western blot developed with α-HeT-A Gag antibody. Lane 1: S2 cells; Lane 2: S2 cells transfected with HeT-A Gag-GFP; Lane 3: S2 cells transfected with HeT-A Gag-Flag; Lane 4: brains of 3rd instar GIII larvae. Both the recombinant and the endogenous HeT-A Gag proteins are detected. (B) Western blot developed with α-TART Pol antibody. Lane 1: extract from stable transfected S2 cells TART-RT-CTAP; Lane 2: extract from brains of 3rd instar GIII larvae. Both the recombinant and the endogenous RT proteins are detected.