Figure 4. Puromycin significantly reduces mammosphere formation in MCF7 cells, without affecting MCF7 cell viability or proliferation.

Increasing concentrations of puromycin inhibit mammosphere formation, using an ER-positive cell line (MCF7). Importantly, puromycin significantly reduces mammosphere formation, with an IC-50 of ~ 0.05 μg/ml. However, mammosphere formation was completed abolished at 0.5 μg/ml. The vehicle-alone control was normalized to one. (*)p <0.05.