Table 5. “Ribosomal Targets” Over-Expressed in Mammospheres are also Transcriptionally Up-regulated in Human Breast Cancer Cells In Vivo (Cancer Epithelia vs. Tumor Stroma).
| Symbol | Gene Description | Fold-Upregulation (Epithelial/Stromal) | P-value |
|---|---|---|---|
| Small Subunit (12) | |||
| RPS27A | 40S ribosomal protein S27A | 4.63 | 1.19E-05 |
| RPS3A | 40S ribosomal protein S3a | 4.59 | 1.35E-05 |
| RPS20 | 40S ribosomal protein S20 | 4.44 | 2.25E-05 |
| RPS6 | 40S ribosomal protein S6 | 4.09 | 7.27E-05 |
| RPS25 | 40S ribosomal protein S25 | 4.05 | 8.29E-05 |
| RPS4X | 40S ribosomal protein S4, X-linked | 3.92 | 1.27E-04 |
| RPS23 | 40S ribosomal protein S23 | 3.56 | 3.92E-04 |
| RPS7 | 40S ribosomal protein S7 | 3.55 | 4.06E-04 |
| RPS3 | 40S ribosomal protein S3 | 3.33 | 7.91E-04 |
| RPS21 | 40S ribosomal protein S21 | 2.75 | 4.00E-03 |
| RPS29 | 40S ribosomal protein S29 | 2.31 | 1.25E-02 |
| RPS9 | 40S ribosomal protein S9 | 1.83 | 3.65E-02 |
| Large Subunit (20) | |||
| RPL7 | 60S ribosomal protein L7 | 5.21 | 1.53E-06 |
| RPL23A | 60S ribosomal protein L23A | 5.13 | 1.99E-06 |
| RPL3 | 60S ribosomal protein L3 | 5.01 | 3.14E-06 |
| RPL10 | 60S ribosomal protein L10 | 4.91 | 4.48E-06 |
| RPLP0 | 60S acidic ribosomal protein P0 | 4.89 | 4.71E-06 |
| RPL14 | 60S ribosomal protein L14 | 4.45 | 2.15E-05 |
| RPL17 | 60S ribosomal protein L17 | 4.15 | 6.00E-05 |
| RPL32 | 60S ribosomal protein L32 | 4.03 | 8.74E-05 |
| RPL6 | 60S ribosomal protein L6 | 4.00 | 9.86E-05 |
| RPL19 | 60S ribosomal protein L19 | 3.97 | 1.05E-04 |
| RPL8 | 60S ribosomal protein L8 | 3.86 | 1.51E-04 |
| RPL9 | 60S ribosomal protein L9 | 3.71 | 2.49E-04 |
| RPL23 | 60S ribosomal protein L23 | 3.56 | 3.92E-04 |
| RPL12 | 60S ribosomal protein L12 | 3.42 | 6.09E-04 |
| RPL36 | 60S ribosomal protein L36 | 3.37 | 6.91E-04 |
| RPL37A | 60S ribosomal protein L37A | 3.13 | 1.42E-03 |
| RPL27 | 60S ribosomal protein L27 | 2.57 | 6.41E-03 |
| RPL18A | 60S ribosomal protein L18A | 2.28 | 1.34E-02 |
| RPL5 | 60S ribosomal protein L5 | 1.86 | 3.45E-02 |
| RPL36AL | 60S ribosomal protein L36A-like | 1.70 | 4.76E-02 |
| Mitochondrial Ribosomal Proteins (2) | |||
| MRPS22 | 28S ribosomal protein S22, mitochondrial | 3.27 | 9.31E-04 |
| MRPL17 | 39S ribosomal protein L17, mitochondrial | 2.94 | 2.38E-03 |
| Translation initiation factors (required for mRNA binding to ribosomes) (4) | |||
| EIF3H | Eukaryotic translation initiation factor 3 subunit H | 4.70 | 9.25E-06 |
| EIF3E | Eukaryotic translation initiation factor 3 subunit E | 3.57 | 3.75E-04 |
| EIF2B4 | Translation initiation factor eIF-2B subunit delta | 3.01 | 1.97E-03 |
| EIF3B | Eukaryotic translation initiation factor 3 subunit B (EIF3S9) | 2.20 | 1.59E-02 |
| Elongation factors (promote delivery of aminoacyl tRNAs to the ribosome) (5) | |||
| EEF2 | Elongation factor 2 | 4.01 | 9.29E-05 |
| EEF1G | Elongation factor 1 gamma | 3.71 | 2.44E-04 |
| TUFM | Elongation factor Tu, mitochondrial | 3.38 | 6.74E-04 |
| EEF1A1 | Elongation factor 1 alpha | 3.16 | 1.30E-03 |
| EEF1D | Elongation factor 1 delta | 2.50 | 7.67E-03 |
| Enzymes for tRNA Synthesis (7) | |||
| RTCB | tRNA-splicing ligase (C22orf28) | 4.58 | 1.37E-05 |
| MARS | methionine-tRNA synthetase | 4.35 | 3.00E-05 |
| EPRS | Bifunctional-tRNA ligase (Glutamate and Proline) | 4.06 | 8.10E-05 |
| DARS | Aspartate--tRNA ligase, cytoplasmic | 3.43 | 5.87E-04 |
| WARS | Tryptophan--tRNA ligase, cytoplasmic | 2.48 | 8.17E-03 |
| SARS | Serine--tRNA ligase, cytoplasmic | 2.15 | 1.81E-02 |
| YARS | Tyrosine--tRNA ligase, cytoplasmic | 1.72 | 4.55E-02 |
-Transcriptional profiling data derived from the analysis of N=28 breast cancer patients are shown, high-lighting the levels of fold-upregulation observed in the epithelial cancer cell compartment (relative to the tumor stroma), and corresponding p-values derived from the analysis of these clinical samples.
-Proteins listed above (50 in total) were all upregulated either in MCF7 or T47D mammospheres (See Tables 1 & 2).