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. 2014 Dec 6;6(7):4615–4632. doi: 10.18632/oncotarget.2652

Figure 5. Enhancement of tumor cells invasiveness after stromal fibroblast interaction.

Figure 5

(A) A375 cells were serum starved for 24 h, seeded in Matrigel-coated upper side of the Boyden chambers and allowed to migrate toward CM derived from HDFs, preincubated for 24 h with NE (10 μM). The last two samples were exposed again to NE (1 μM) for additional 24 h, ***P < 0.0001 vs standard medium. (B) A375 cells were stained with Quantum Dot to render them fluorescent. After 24 h cells were serum starved for additional 24 h and seeded in the upper side of Matrigel-coated and then allowed to invade CM derived from HDFs, preincubated in the presence or absence of NE (10 μM) and/or βARs antagonist ICI 118-551 (1 μM), SR59230A (10 μM) for 24 h. Bar graphs show the mean ± SD of three independent ***P < 0.0001 vs CM from HDFs-NE stimulated.