Skip to main content
. 2015 Mar 5;6(7):4773–4789. doi: 10.18632/oncotarget.3000

Figure 2. Binding of SPIN1 to H3K4me3 is required for proliferation and survival of liposarcoma cells.

Figure 2

(A, B) Proliferation of T778 cells transfected with control siRNA (siCtrl) or siRNA directed against SPIN1 [siSPIN1(1)] and expression plasmid for RNAi-resistant, wildtype or mutant SPIN1 (rr-SPIN1 or rr-SPIN1 F141A, respectively). Growth curves (A) and slopes of exponential growth phases (B) are shown. (C, F) Western blot analysis of SPIN1 expression in T778 cells transfected with siRNA and SPIN1 expression plasmid as indicated. An asterisk marks exogenous SPIN1 having a higher molecular weight than endogenous SPIN1 due to the presence of a tag. α-Tubulin was used as a loading control. In (F), SPIN1 and SPIN1 F141A expression in stably transfected T778 cells was induced by doxycycline (Dox). (D) Caspase 3 activity in T778 cells transfected with siRNA and SPIN1 expression plasmid as indicated. (E) Determination of EC50 values for nutlin-3a-induced apoptosis of T778 cells. Cells were transfected with siRNA and SPIN1 expression plasmid as indicated. Expression of exogenous SPIN1 was induced by doxycycline. EC50 values were calculated from treatment of cells with different concentrations of nutlin-3a. (B, D, E) Error bars represent +/– SEM, **p < 0.01, ***p < 0.001.