Figure 2. DNA damage and telomere dysfunction mediated by 5-AZA in AML cells.

KG1A and HEL cells were treated with 5-AZA at 2.0 μM for 72 hours and then analyzed for 53-BP1 foci and co-localization of telomere signals with 53-BP1 foci using Immuno-FISH. Red and Green: 53-BP1 foci and telomere signals, respectively. Yellow: Co-localization of 53-BP1 foci and telomere signals. Shown is the representative of three independent experiments.