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. 2015 Feb 17;6(7):4888–4900. doi: 10.18632/oncotarget.2917

Figure 5. Down-regulation of TERT mRNA expression and telomerase activity in 5-AZA-treated AML cells.

Figure 5

KG1A and HEL cells were treated with 5-AZA for 72 hours, and TERT mRNA (A) and telomerase activity (B) were then determined using qPCR and Telomerase ELISA kit, respectively. Both TERT mRNA and telomerase activity in 5-AZA-treated cells were expressed as the percentage of those in control cells. * denotes P < 0.05. (C) The diminished TERT mRNA expression in 5-AZA-treated primary leukemic cells isolated from 5 patients with AML. Leukemic cells were treated with 5-AZA at 5.0 μM for 72 hours then analysed for TERT mRNA expression using qPCR. The level of TERT mRNA in the treated cells was expressed as that in control cells. The values are means ± SD. Three independent experiments were performed.